Gram staining is a exceptional technique which is used to stain bacteria. This technique was developed past times Christian Gram inwards 1884. The stain stain used inwards Gram staining is called Gram stain. Chemically Gram stain is a weakly element of group I solution of crystal purple or gentian violet.
On the footing of prison theatre cellular telephone wall construction together with its staining might with Gram stain, bacteria are grouped into ii categories. They are Gram positive bacteria together with Gram negative bacteria
On the footing of prison theatre cellular telephone wall construction together with its staining might with Gram stain, bacteria are grouped into ii categories. They are Gram positive bacteria together with Gram negative bacteria
Cell wall of Gram positive bacteria | Cell wall of Gram negative bacteria |
Cell wall is unmarried layered together with primarily made upwards of peptidoglycan | Cell wall is double layered together with amongst an outer membrane exterior to Peptidoglycan layer |
Gram positive prison theatre cellular telephone wall retain the principal stain of Gram staining (crystal violet) together with appear majestic later on alcohol handling | Gram positive prison theatre cellular telephone wall lose the principal stain of Gram staining (crystal violet) later on alcohol treatment; together with appear pinkish amongst counter stain (safranin) |
Thick peptidoglycan layer of close xx to lxxx nm | Peptidoglycan layer sparse together with unmarried layered (about v to 10 nm thick) |
Periplasmic infinite is absent | Periplasmic space* is absent |
Outer membrane is absent | Outer membrane is acquaint exterior to Peptidoglycan layer (about 7.5 to 10 nm thick) |
Teichoic acid is present | Generally Teichoic acid is absent |
Generally **Porins are absent | Porins are present |
Very depression lipid content (2-5%) | Very high lipid content (15-20%) |
Generally Lipopolysaccharide (LPS) is absent** | Lipopolysaccharide (LPS) is present |
Lysozyme degrade peptidoglycan wall movement lysis of prison theatre cellular telephone (sensitive to lysozyme) | Lysozyme cannot degrade Gram negative bacteria every bit Peptidoglycan is protected past times outer membrane (resistant to lysozyme) |
| Example of Gram Negative Bacteria |
* The share betwixt cytoplasmic together with outer membrane of the Gram-negative bacteria is called the periplasmic space
*Porins attain large, open, water-filled channels that allow the influx of normal, hydrophilic food molecules together with the efflux of waste matter products but exclude many antibiotics together with inhibitors
**only i Gram-positive organism, Listeria monocytogenes, has been institute to comprise LPS
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