Mr X Ten Differences Betwixt Southern Blotting Too Western Blotting

Southern blotting: The Southern blot is used to let on the presence of a item deoxyribonucleic acid fragment inward a sample.The technique was developed past times E.M. Southern inward 1975.

Western Blotting: Technique for detecting specific proteins separated past times electrophoresis past times role of labelled antibodies. The technique was developed past times Towbin et al inward 1979.

 Southern Blotting VS Western Blotting

Southern Blotting	Western Blotting
Technique for the identification of specific deoxyribonucleic acid inward a sample	Technique for the identification of specific poly peptide inward a sample
Developed past times E.M. Southern hence called “ Southern”	The term “Western” has no scientific significance but a misnomer to check amongst Southern blotting
Principle is Hybridization; the physical care for of forming a double-stranded deoxyribonucleic acid molecule betwixt a single-stranded deoxyribonucleic acid probe too a single-stranded target DNA. (Southern Blotting Steps)	Principle: Antigen-antibody interaction, an immunodetection method (Refer: Western Blotting)
Probe used is unmarried stranded deoxyribonucleic acid or sometimes RNA	Probe used is antibodies specifically targeted against epitopes of antigens
DNA-DNA hybridization or RNA-DNA hybridization	Antibody-antigen complex formation
DNA fragments separated past times Agarose gel electrophoresis	Proteins separated past times SDS-PAGE (Sodium dodecyl sulphate-PolyAcrylamide Gel Electrophoresis)
DNA is denatured amongst an alkali metal solution such every bit NaOH earlier blotting. This causes the double stranded to larn single-stranded.	SDS denatures poly peptide too imparts an overall negative charge
No such footstep every bit blocking (SouthernBlotting- Principle, Procedure too Applications)	Blocking nonspecific antibody sites on the nitrocellulose newspaper amongst bovine serum albumin (BSA) or milk powder
ssDNA or rarely RNA every bit probe	Probe include top dog antibody followed past times labelled secondary antibody
Common labeling methods include radio labeling or fluorescent labeling or role of chromogenic dyes. Southern vs Northern Blotting (Video)	Common labeling methods include horseradish peroxidase-anti-Ig conjugate or formation of a diaminobenzidine (DAB) precipitate (chromogenic dye), radiolabelling or role of fluorescently labelled secondary antibody.